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1.
Int J Biol Macromol ; 257(Pt 1): 128604, 2024 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-38056729

RESUMO

Nowadays, various high-performance electromagnetic interference (EMI) shielding materials have enormous application potential in electronic field. However, traditional EMI shielding materials often have high conductivity, resulting in the serious mismatch between the impedance of the material surface and the free space, which will cause a large amount of reflection of electromagnetic (EM) waves, leading to secondary reflection pollution. In this paper, we report a novel flexible EMI shielding composite film with extremely low reflection loss and efficient EM wave absorption, which was prepared by assisted self-assembly based on simple vacuum filtration using carboxymethyl cellulose as the matrix and MWCNT@Fe3O4 synthesized by chemical coprecipitation as the composite functional filler. By adjusting the Fe3O4 coating degree of MWCNTs in the filler, the composite film achieved the construction of a conductive network with high Fe3O4 content. Benefit by the good adaptability of conductivity and magnetic permeability, the composite film has good impedance matching ability and microwave absorption performance. The reflection loss of the composite film with the thickness of 28 µm in the X-band was only 0.23 dB, accounting for 1.7 % of the total loss. This work provides new insights for the development of EMI materials and effective mitigation secondary EM wave reflection pollution.


Assuntos
Absorção de Radiação , Carboximetilcelulose Sódica , Condutividade Elétrica , Impedância Elétrica , Eletrônica , Excipientes
2.
ACS Nano ; 17(20): 19853-19864, 2023 10 24.
Artigo em Inglês | MEDLINE | ID: mdl-37812400

RESUMO

Due to the immunosuppressive tumor microenvironment (TME) and weak radiation absorption, the immune response triggered by radiation therapy (RT) is limited. Herein, a core-shell nanosensitizer UiO@MnS (denoted as UM) was genuinely constructed for the amplification of RT efficacy and induction of immunogenicity via integrating MnS-reprogrammed TME with Hf-based UiO-sensitized RT. The acid-sensitive MnS would produce H2S under acidic TME to improve oxygenation through inhibition mitochondrial respiration and reducing metabolic oxygen consumption, leading to decreased HIF-1α expression and enhanced radiosensitization. In addition, the generated H2S inhibited the catalase activity to increase the H2O2 level, which subsequently enhanced the Mn2+-mediated Fenton-like reaction, resulting in G2/M cell cycle arrest to improve the cellular sensitivity for radiation. This impressive tumor oxygenation, cell cycle arrest, and radiosensitization procedure boosted RT efficacy and resulted in strong antitumor immunogenicity. Taken together, combining the immunosuppressive TME modulation with a sensitizing radiation strategy shows great promise for magnifying immunogenic RT outputs.


Assuntos
Peróxido de Hidrogênio , Neoplasias , Humanos , Microambiente Tumoral , Absorção de Radiação , Ciclo Celular , Divisão Celular , Imunossupressores , Neoplasias/radioterapia , Linhagem Celular Tumoral
3.
Sci Bull (Beijing) ; 68(11): 1195-1212, 2023 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-37179235

RESUMO

Polymer composites have essential applications in electronics due to their versatility, stable performance, and processability. However, with the increasing miniaturization and high power of electronics in the 5G era, there are significant challenges related to heat accumulation and electromagnetic wave (EMW) radiation in narrow spaces. Traditional solutions involve using either thermally conductive or EMW absorbing polymer composites, but these fail to meet the demand for multi-functional integrated materials in electronics. Therefore, designing thermal conduction and EMW absorption integrated polymer composites has become essential to solve the problems of heat accumulation and electromagnetic pollution in electronics and adapt to its development trend. Researchers have developed different approaches to fabricate thermal conduction and EMW absorption integrated polymer composites, including integrating functional fillers with both thermal conduction and EMW absorption functions and innovating processing methods. This review summarizes the latest research progress, factors that affect performance, and the mechanisms of thermal conduction and EMW absorption integrated polymer composites. The review also discusses problems that limit the development of these composites and potential solutions and development directions. The aim of this review is to provide references for the development of thermal conduction and EMW absorption integrated polymer composites.


Assuntos
Absorção de Radiação , Eletrônica , Condutividade Elétrica , Polímeros , Radiação Eletromagnética
4.
Int J Mol Sci ; 24(10)2023 May 11.
Artigo em Inglês | MEDLINE | ID: mdl-37239958

RESUMO

In this study, we successfully prepared core-shell heterostructured nanocomposites (Fe NWs@SiO2), with ferromagnetic nanowires (Fe NWs) as the core and silica (SiO2) as the shell. The composites exhibited enhanced electromagnetic wave absorption and oxidation resistance and were synthesized using a simple liquid-phase hydrolysis reaction. We tested and analyzed the microwave absorption properties of Fe NWs@SiO2 composites with varied filling rates (mass fractions of 10 wt%, 30 wt%, and 50 wt% after mixing with paraffin). The results showed that the sample filled with 50 wt% had the best comprehensive performance. At the matching thickness of 7.25 mm, the minimum reflection loss (RLmin) could reach -54.88 dB at 13.52 GHz and the effective absorption bandwidth (EAB, RL < -10 dB) could reach 2.88 GHz in the range of 8.96-17.12 GHz. Enhanced microwave absorption performance of the core-shell structured Fe NWs@SiO2 composites could be attributed to the magnetic loss of the composite, the core-shell heterogeneous interface polarization effect, and the small-scale effect induced by the one-dimensional structure. Theoretically, this research provided Fe NWs@SiO2 composites with highly absorbent and antioxidant core-shell structures for future practical applications.


Assuntos
Nanofios , Dióxido de Silício , Absorção de Radiação , Ferro , Micro-Ondas
5.
Biochim Biophys Acta Biomembr ; 1865(4): 184136, 2023 04.
Artigo em Inglês | MEDLINE | ID: mdl-36746311

RESUMO

A recent study provided experimental evidence of inactivation of viral activity after radio-frequency (RF) exposures in the 6-12 GHz band that was hypothesized to be caused by vibrations of an acoustic dipole mode in the virus that excited the viral membrane to failure. Here, we develop an atomic-scale molecular dynamics (MD) model of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral surface to estimate the electric fields necessary to rupture the viral membrane via dipole shaking of the virus. We computed the absorption spectrum of the system via unbiased MD simulations and found no particular strong absorption in the GHz band. We investigated the mechanical resiliency of the viral membrane by introducing uniaxial strains in the system and observed no pore formation in the membrane for strains up to 50%. Because the computed absorption spectrum was found to be essentially flat, and the strain required to break the viral membrane was >0.5, the field strength associated with rupture of the virus was greater than the dielectric breakdown value of air. Thus, RF disinfection of enveloped viruses would occur only once sufficient heat was transferred to the virus via a thermal mechanism and not by direct action (shaking) of the RF field oscillations on the viral membrane.


Assuntos
Simulação de Dinâmica Molecular , Ondas de Rádio , SARS-CoV-2 , Envelope Viral , SARS-CoV-2/química , SARS-CoV-2/efeitos da radiação , Propriedades de Superfície , Envelope Viral/química , Envelope Viral/efeitos da radiação , Desinfecção , Absorção de Radiação
6.
Photochem Photobiol Sci ; 22(1): 47-58, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36112308

RESUMO

Pure (TiO2) and 0.1 nominal atomic percent of palladium-modified TiO2 (Pd-TiO2) films were synthesized via a sol-gel method and compared through their physicochemical properties and photocatalytic activity in the degradation of an emerging contaminant, 17-α-ethinylestradiol (EE2). The activity of the films was studied using a continuous flow, planar microreactor under simulated sunlight. Catalysts characterization included X-ray diffraction, UV-Visible diffuse reflectance and transmittance spectroscopy, atomic force microscopy, transmission electron microscopy, Raman spectroscopy, N2 physisorption analysis, and X-ray photoelectron spectroscopy. The modification of TiO2 with palladium confined the size of anatase phase crystallites, increased the specific surface area and improved radiation absorption. PdO domains on TiO2 were observed. In all the tested conditions, higher conversion of EE2 was achieved with the Pd-TiO2 film compared with the TiO2 film, presenting an 80% increase in the reaction rate. The performance of the catalytic films was also assessed by the calculation of two efficiency parameters: radiation absorption efficiency and quantum efficiency of reaction. The Pd-TiO2 film showed a notable enhancement of the absorption of the incident radiation and a more efficient utilization of the absorbed photons to degrade the target pollutant.


Assuntos
Absorção de Radiação , Paládio , Microscopia Eletrônica de Transmissão , Titânio/química , Catálise
7.
Int J Mol Sci ; 23(23)2022 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-36498829

RESUMO

Carbon-based carbides have attracted tremendous attention for electromagnetic energy attenuation due to their adjustable dielectric properties, oxidation resistance, and good chemical stability. Herein, we reasonably regulate the growth of dopamine hydrochloride on the surface of the Mo-glycerate (Mo-GL) microsphere and then transform the resultant Mo-polydopamine (Mo-PD) microsphere into a dual-shell Mo2C/C (DS-Mo2C/C) microsphere in a high-temperature pyrolysis process under an inert atmosphere. It is found that the pyrolysis temperature plays an important role in the graphitization degree of the carbon matrix and internal architecture. The fabrication of a dual-shell structure can be propitious to the optimization of impedance matching, and the introduction of Mo2C nanoparticles also prompts the accumulation of polarization loss. When the pyrolysis temperature reaches 800 °C, the optimized composite of DS-Mo2C/C-800 exhibits good EM absorption performance in the frequency range of 2.0-18.0 GHz. DS-Mo2C/C-800's qualified bandwidth can reach 4.4 GHz at a matching thickness of 1.5 mm, and the integrated qualified bandwidth (QBW) even exceeds 14.5 GHz with a thickness range of 1.5-5.0 mm. The positive effects of the dual-shell structure and Mo2C nanoparticles on EM energy attenuation may render the DS-Mo2C/C microsphere as a promising candidate for lightweight and broad bandwidth EM absorption materials in the future.


Assuntos
Absorção de Radiação , Pirólise , Microesferas , Carbono , Atmosfera
8.
Electromagn Biol Med ; 41(2): 184-200, 2022 Apr 03.
Artigo em Inglês | MEDLINE | ID: mdl-35352614

RESUMO

A strong evidence of the effects of radiation absorption on the living community together with a better solution to reduce the radiation intensity without compromising the usage of wireless communication systems is presented. This study analyses the radiation effects on living things and validates the proposed techniques for specific absorption rate (SAR) value reduction at 2.45 GHz. To reduce these radiation impacts on the living community, proper shielding from the radiation and effectively reorienting antenna radiation patterns are the solutions suggested. An analogous antenna configuration in wireless communication systems - a coplanar waveguide fed loop antenna is considered and an open loop resonator (OLR) optimized in ANSYS HFSS at 2.45 GHz is incorporated on the back side of the proposed antenna for achieving SAR value reduction. Theoretical and experimental validation is carried out by measuring the variation in absorption power on each vegetable sample using vector network analyzer E5080A. The existence of OLR on the back side of the antenna reduces the absorption power upto 2 dB. From experimental validation, the proposed technique provides 88% to 98% reduction in SAR value when tested in each sample. Along with this OLR exhibits the capability to enhance the shielding characteristics to the controlled environment of experimental setup for analyzing the stages of seed germination, which helps in reducing the reported radiation effects and growth retardation. The proposed method of EMR reduction with miniaturized planar resonator can be effectively used in the communication systems operating at 2.45 GHz for creating a reduced radiation environment.


Assuntos
Absorção de Radiação , Tecnologia sem Fio , Radiação Eletromagnética , Desenho de Equipamento
9.
Int J Mol Sci ; 24(1)2022 Dec 28.
Artigo em Inglês | MEDLINE | ID: mdl-36613944

RESUMO

In this work, a novel core-shell structure material, NiFe layered double hydroxide (NiFe LDH) loaded on SiO2 microspheres (SiO2@NiFe LDH), was synthesized by a one-step hydrothermal method, and the spontaneous electrostatic self-assembly process. The morphology, structure, and microwave absorption properties of SiO2@NiFe LDH nanocomposites with different NiFe element ratios were systematically investigated. The results show that the sample of SiO2@NiFe LDH-3 nanocomposite has excellent microwave absorption properties. It exhibits broadband effective absorption bandwidth (RL < −10 dB) of 8.24 GHz (from 9.76 GHz to 18.0 GHz) and the reflection loss is −53.78 dB at the matched thickness of 6.95 mm. It is expected that this SiO2@NiFe-LDH core-shell structural material can be used as a promising non-precious, metal-based material microwave absorber to eliminate electromagnetic wave contamination.


Assuntos
Absorção de Radiação , Dióxido de Silício , Contaminação de Medicamentos , Microesferas , Micro-Ondas
10.
Exp Eye Res ; 213: 108842, 2021 12.
Artigo em Inglês | MEDLINE | ID: mdl-34793829

RESUMO

Avoiding damage of the endothelial cells, especially in thin corneas, remains a challenge in corneal collagen crosslinking (CXL). Knowledge of the riboflavin gradients and the UV absorption characteristics after topical application of riboflavin in concentrations ranging from 0.1% to 0.5% could optimize the treatment. In this study, we present a model to calculate the UV-intensity depending on the corneal thickness. Ten groups of de-epithelialized porcine corneas were divided into 2 subgroups. Five groups received an imbibition of 10 min and the other five groups for 30 min. The applied riboflavin concentrations were 0.1%, 0.2%, 0.3%, 0.4% and 0.5% diluted in a 15% dextran solution for each subgroup. After the imbibition process, two-photon fluorescence microscopy was used to determine fluorescence intensity, which was compared to samples after saturation, yielding the absolute riboflavin concentration gradient of the cornea. The extinction coefficient of riboflavin solutions was measured using a spectrophotometer. Combining the obtained riboflavin concentrations and the extinction coefficients, a depth-dependent UV-intensity profile was calculated for each group. With increasing corneal depth, the riboflavin concentration decreased for all imbibition solutions and application times. The diffusion coefficients of 10 min imbibition time were higher than for 30 min. A higher RF concentration and a longer imbibition time resulted in higher UV-absorption and a lower UV-intensity in the depth of the cornea. Calculated UV-transmission was 6 percentage points lower compared to the measured transmission. By increasing the riboflavin concentration of the imbibition solution, a substantially higher UV-absorption inside the cornea is achieved. This offers a simple treatment option to control the depth of crosslinking e.g. in thin corneas, resulting in a lower risk of endothelial damage.


Assuntos
Absorção de Radiação/efeitos dos fármacos , Substância Própria/metabolismo , Fármacos Fotossensibilizantes/farmacocinética , Riboflavina/farmacocinética , Raios Ultravioleta , Administração Oftálmica , Animais , Paquimetria Corneana , Substância Própria/efeitos da radiação , Reagentes de Ligações Cruzadas , Microscopia de Fluorescência por Excitação Multifotônica , Soluções Oftálmicas , Fotoquimioterapia , Fármacos Fotossensibilizantes/administração & dosagem , Riboflavina/administração & dosagem , Suínos
11.
Sci Rep ; 11(1): 18365, 2021 09 15.
Artigo em Inglês | MEDLINE | ID: mdl-34526535

RESUMO

The physicochemical and antioxidant properties of seven carotenoids: antheraxanthin, ß-carotene, neoxanthin, peridinin, violaxanthin, xanthrophyll and zeaxanthin were studied by theoretical means. Then the Optoelectronic properties and interaction of chlorophyll-carotenoid complexes are analysed by TDDFT and IGMPLOT. Global reactivity descriptors for carotenoids and chlorophyll (Chla, Chlb) are calculated via conceptual density functional theory (CDFT). The higher HOMO-LUMO (HL) gap indicated structural stability of carotenoid, chlorophyll and chlorophyll-carotenoid complexes. The chemical hardness for carotenoids and Chlorophyll is found to be lower in the solvent medium than in the gas phase. Results showed that carotenoids can be used as good reactive nucleophile due to lower µ and ω. As proton affinities (PAs) are much lower than the bond dissociation enthalpies (BDEs), it is anticipated that direct antioxidant activity in these carotenoids is mainly due to the sequential proton loss electron transfer (SPLET) mechanism with dominant solvent effects. Also lower PAs of carotenoid suggest that antioxidant activity by the SPLET mechanism should be a result of a balance between proclivities to transfer protons. Reaction rate constant with Transition-State Theory (TST) were estimated for carotenoid-Chlorophyll complexes in gas phase. Time dependent Density Functional Theory (TDDFT) showed that all the chlorophyll (Chla, Chlb)-carotenoid complexes show absorption wavelength in the visible region. The lower S1-T1 adiabatic energy gap indicated ISC transition from S1 to T1 state.


Assuntos
Antioxidantes/química , Carotenoides/química , Clorofila/química , Elétrons , Absorção de Radiação , Antioxidantes/efeitos da radiação , Carotenoides/efeitos da radiação , Clorofila/efeitos da radiação , Oxigênio/química
12.
Sci Rep ; 11(1): 17135, 2021 08 24.
Artigo em Inglês | MEDLINE | ID: mdl-34429467

RESUMO

The 3D printing of fluorescent materials could help develop, validate, and translate imaging technologies, including systems for fluorescence-guided surgery. Despite advances in 3D printing techniques for optical targets, no comprehensive method has been demonstrated for the simultaneous incorporation of fluorophores and fine-tuning of absorption and scattering properties. Here, we introduce a photopolymer-based 3D printing method for manufacturing fluorescent material with tunable optical properties. The results demonstrate the ability to 3D print various individual fluorophores at reasonably high fluorescence yields, including IR-125, quantum dots, methylene blue, and rhodamine 590. Furthermore, tuning of the absorption and reduced scattering coefficients is demonstrated within the relevant mamalian soft tissue coefficient ranges of 0.005-0.05 mm-1 and 0.2-1.5 mm-1, respectively. Fabrication of fluorophore-doped biomimicking and complex geometric structures validated the ability to print feature sizes less than 200 µm. The presented methods and optical characterization techniques provide the foundation for the manufacturing of solid 3D printed fluorescent structures, with direct relevance to biomedical optics and the broad adoption of fast manufacturing methods in fluorescence imaging.


Assuntos
Resinas Acrílicas/química , Impressão Tridimensional/instrumentação , Absorção de Radiação , Resinas Acrílicas/efeitos da radiação , Fluorescência , Corantes Fluorescentes/química
13.
Sci Rep ; 11(1): 15293, 2021 07 27.
Artigo em Inglês | MEDLINE | ID: mdl-34315976

RESUMO

The optical absorption coefficient of culture media is critical for the survival analysis of pathogens under optical irradiation. The quality of the results obtained from experiments relies on the optical analysis of the spatial distribution of fluence which also depends on the geometry of the sample. In this contribution, we consider both the geometrical shape and the culture medium's absorption coefficient to evaluate how the spatial distribution of optical radiation affects pathogens/viruses. In this work, we exposed SARS-CoV-2 to UV-C radiation ([Formula: see text] = 254 nm) and we calculated-considering the influence of the optical absorption of the culture medium-a characteristic inactivation fluence of [Formula: see text] = 4.7 J/m2, or an equivalent 10% survival (D90 dose) of 10.8 J/m2. Experimentally, we diluted the virus into sessile drops of Dulbecco's Modified Eagle Medium to evaluate pathogen activity after controlled doses of UV irradiation. To validate the optical absorption mode, we carried out an additional experiment where we varied droplet size. Our model-including optical absorption and geometrical considerations-provides robust results among a variety of experimental situations, and represents our experimental conditions more accurately. These results will help to evaluate the capability of UV disinfecting strategies applied to a variety of everyday situations, including the case of micro-droplets generated by respiratory functions.


Assuntos
Absorção de Radiação , Meios de Cultura , SARS-CoV-2/fisiologia , SARS-CoV-2/efeitos da radiação , Raios Ultravioleta , Inativação de Vírus/efeitos da radiação , Desinfecção
14.
Int J Mol Sci ; 22(13)2021 Jun 26.
Artigo em Inglês | MEDLINE | ID: mdl-34206765

RESUMO

A [H2DPEphos][MnX4] [X = Br, Cl] tetrahalomanganates(II) with P,P'-diprotonated bis[2-(diphenylphosphino)phenyl]ether cation has been designed and investigated in photophysics and EPR terms. The complexes exhibit a green luminescence resulted from the Mn(II) d-d transitions (4T1→6A1) with the wavelength-excitation dependence of the quantum yield. The solid [H2DPEphos][MnBr4] complex exhibits a bright green phosphorescence (λmax = 515 nm) with the high luminescence quantum yield depending on the excitation energy whereas the solid [H2DPEphos][MnCl4] complex exhibits a very weak phosphorescence (λmax = 523 nm). The unexpected shorter luminescence lifetime for the [H2DPEphos][MnCl4] than for the [H2DPEphos][MnBr4] at 300 K can be a result of the higher non-radiative relaxation contribution. On the one hand, the non-covalent PH…X(Mn) interactions quench the manganese(II) luminescence. On the other hand, the PH…X(Mn) interactions are a pathway of the excitation transfer from [H2DPEphos]2+ to [MnX4]2-.


Assuntos
Substâncias Luminescentes/química , Compostos de Manganês/química , Marcadores de Spin , Absorção de Radiação , Espectroscopia de Ressonância de Spin Eletrônica/métodos , Prótons , Raios Ultravioleta
15.
Sci Rep ; 11(1): 13930, 2021 07 06.
Artigo em Inglês | MEDLINE | ID: mdl-34230618

RESUMO

The activity of radioiodine (131I) used in adjuvant therapy for thyroid cancer ranges between 30 mCi (1.1 GBq) and 150 mCi (5.5 GBq). Dosimetry based on Marinelli's formula, taking into consideration the absorbed dose in the postoperative tumour bed (D) should systematise the determination of 131I activity. Retrospective analysis of 57 patients with differentiated thyroid cancer (DTC) after thyreidectomy and adjuvant 131I therapy with the fixed activity of 3.7 GBq. In order to calculate D from Marinelli's formula, the authors took into account, among other things, repeated dosimetry measurements (after 6, 24, and 72 h) made during scintigraphy and after administration of the therapeutic activity or radioiodine. In 75% of the patients, the values of D were > 300 Gy (i.e. above the value recommended by current guidelines). In just 16% of the patients, the obtained values fell between 250 and 300 Gy, whereas in 9% of the patients, the value of D was < 250 Gy. The therapy was successful for all the patients (stimulated Tg < 1 ng/ml and 131I uptake < 0.1% in the thyroid bed in follow-up examination). Dosimetry during adjuvant 131I therapy makes it possible to diversify the therapeutic activities of 131I in order to obtain a uniform value of D.


Assuntos
Radioisótopos do Iodo/uso terapêutico , Dosagem Radioterapêutica , Neoplasias da Glândula Tireoide/radioterapia , Absorção de Radiação , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Pescoço/diagnóstico por imagem , Neoplasias da Glândula Tireoide/diagnóstico por imagem , Distribuição Tecidual , Tomografia Computadorizada de Emissão de Fóton Único , Tomografia Computadorizada por Raios X
16.
NMR Biomed ; 34(7): e4525, 2021 07.
Artigo em Inglês | MEDLINE | ID: mdl-33955061

RESUMO

PURPOSE: To investigate inter-subject variability of B1+ , SAR and temperature rise in a database of human models using a local transmit array for 7 T cardiac imaging. METHODS: Dixon images were acquired of 14 subjects and segmented in dielectric models with an eight-channel local transmit array positioned around the torso for cardiac imaging. EM simulations were done to calculate SAR distributions. Based on the SAR distributions, temperature simulations were performed for exposure times of 6 min and 30 min. Peak local SAR and temperature rise levels were calculated for different RF shim settings. A statistical analysis of the resulting peak local SAR and temperature rise levels was performed to arrive at safe power limits. RESULTS: For RF shim vectors with random phase and uniformly distributed power, a safe average power limit of 35.7 W was determined (first level controlled mode). When RF amplitude and phase shimming was performed on the heart, a safe average power limit of 35.0 W was found. According to Pennes' model, our numerical study suggests a very low probability of exceeding the absolute local temperature limit of 40 °C for a total exposure time of 6 min and a peak local SAR of 20 W/kg. For a 30 min exposure time at 20 W/kg, it was shown that the absolute temperature limit can be exceeded in the case where perfusion does not change with temperature. CONCLUSION: Safe power constraints were found for 7 T cardiac imaging with an eight-channel local transmit array, while considering the inter-subject variability of B1+ , SAR and temperature rise.


Assuntos
Absorção de Radiação , Coração/diagnóstico por imagem , Imageamento por Ressonância Magnética , Temperatura , Adulto , Simulação por Computador , Campos Eletromagnéticos , Humanos , Pessoa de Meia-Idade , Modelos Biológicos
17.
Int J Mol Sci ; 22(5)2021 Mar 06.
Artigo em Inglês | MEDLINE | ID: mdl-33800802

RESUMO

Multiphoton microscopy has recently passed the milestone of its first 30 years of activity in biomedical research. The growing interest around this approach has led to a variety of applications from basic research to clinical practice. Moreover, this technique offers the advantage of label-free multiphoton imaging to analyze samples without staining processes and the need for a dedicated system. Here, we review the state of the art of label-free techniques; then, we focus on two-photon autofluorescence as well as second and third harmonic generation, describing physical and technical characteristics. We summarize some successful applications to a plethora of biomedical research fields and samples, underlying the versatility of this technique. A paragraph is dedicated to an overview of sample preparation, which is a crucial step in every microscopy experiment. Afterwards, we provide a detailed review analysis of the main quantitative methods to extract important information and parameters from acquired images using second harmonic generation. Lastly, we discuss advantages, limitations, and future perspectives in label-free multiphoton microscopy.


Assuntos
Microscopia de Fluorescência por Excitação Multifotônica/métodos , Absorção de Radiação , Anisotropia , Análise de Fourier , Microscopia de Polarização/métodos , Microtomia/métodos , Imagem Óptica/métodos , Fotodegradação , Fótons , Microscopia de Geração do Segundo Harmônico/métodos , Manejo de Espécimes/métodos , Fixação de Tecidos/métodos , Análise de Ondaletas
18.
Int J Biol Macromol ; 182: 796-805, 2021 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-33865890

RESUMO

In this study, a high molecular weight arabinoxylan (AX, Mw: 694 kDa) from wheat bran was alkaline extracted and covalently linked with Catechin (CA) by free radical catalytic reaction. Comparing to AX, arabinoxylan-catechin (AX-CA) conjugates demonstrated an extra UV-vis absorption peak at 274 nm, a new FT-IR absorption band at 1516 cm-1 and new proton signals at 6.5-7.5 ppm, which all confirmed the covalently linked structure. Grafting CA onto AX not only decreased the molecular weight, thermal stability and apparent viscosity of AX, but also enhanced its inhibition effects on starch digestibility in vitro. The in vitro fermentation test with pig feces showed that the degradation & utilization rate of AX, the total short-chain fatty acid (SCFA) and acetic acid levels produced all were significantly delayed after grafting. This study provided a novel approach to synthesize AX-CA conjugates that could be a novel dietary fiber of enhanced functional/bioactive properties using in the fields of functional foods and medicine.


Assuntos
Catequina/química , Nanoconjugados/química , Xilanos/química , Absorção de Radiação , Ácido Acético/metabolismo , Animais , Catequina/metabolismo , Ácidos Graxos Voláteis/metabolismo , Fermentação , Temperatura Alta , Hidrólise , Suínos , Triticum/química , Viscosidade , Xilanos/metabolismo
19.
Radiat Res ; 196(5): 535-546, 2021 11 01.
Artigo em Inglês | MEDLINE | ID: mdl-33667298

RESUMO

There is an unmet need to provide medical personnel with a Food and Drug Administration (FDA)-approved biodosimetry method for quantifying individualized absorbed dose response to inform treatment decisions for a very large patient population potentially exposed to ionizing radiation in the event of a nuclear incident. Validation of biodosimetry devices requires comparison of absorbed dose estimates to delivered dose as an indication of accuracy; however, comparison to delivered dose does not account for biological variability or an individual's radiosensitivity. As there is no FDA-cleared gene-expression-based biodosimetry method for determining biological response to radiation, results from accuracy comparisons to delivered dose yield relatively wide tolerance intervals or uncertainty. The Arizona State University Biodesign Institute is developing a high-throughput, automated real-time polymerase chain reaction (RT-PCR)-based biodosimetry system that provides absorbed dose estimates for patients exposed to 0-10 Gy from blood collected 1-7 days postirradiation. While the absorbed dose estimates result from a calibration against the actual exposed dose, the reported dose estimate is a measure of response to absorbed dose based on the exposure models used in developing the system. A central concern with biodosimetry test evaluation is how variability in the dose estimate results could affect medical decision-making, and if the biodosimetry test system performance is quantitatively sufficient to inform effective treatment. A risk:benefit analysis of the expected system performance in the proposed intended use environment was performed to address the potential medical utility of this biodosimetry system. Uncertainty analysis is based on biomarker variability in non-human primate (NHP) models. Monte Carlo simulation was employed to test multiple groups of biomarkers and their potential variation in response to determine uncertainty associated with dose estimate results. Dose estimate uncertainty ranges from ±1.2-1.7 Gy depending on the exposure dose over a range of 2-10 Gy. The risk:benefit of individualized absorbed dose estimates within the context of medical interventions after a nuclear incident is considered and the application of the biodosimetry system is evaluated in this framework. NHP dose-response relationships, as measured by clinical outcome end points, show expected biological and radiosensitivity responses in the primate populations tested and corroborate the biological variability observed in the reported absorbed dose estimate. Performance is examined in relationship to current clinical management and treatment recommendations, with evaluation of potential patient risk in over- and underestimating absorbed dose.


Assuntos
Exposição à Radiação , Absorção de Radiação , Doses de Radiação , Radiometria
20.
Nat Commun ; 12(1): 455, 2021 01 19.
Artigo em Inglês | MEDLINE | ID: mdl-33469005

RESUMO

The technology of magnetic resonance imaging is developing towards higher magnetic fields to improve resolution and contrast. However, whole-body imaging at 7 T or even higher flux densities remains challenging due to wave interference, tissue inhomogeneities, and high RF power deposition. Nowadays, proper RF excitation of a human body in prostate and cardiac MRI is only possible to achieve by using phased arrays of antennas attached to the body (so-called surface coils). Due to safety concerns, the design of such coils aims at minimization of the local specific absorption rate (SAR), keeping the highest possible RF signal in the region of interest. Most previously demonstrated approaches were based on resonant structures such as e.g. dipoles, capacitively-loaded loops, TEM-line sections. In this study, we show that there is a better compromise between the transmit signal [Formula: see text] and the local SAR using non-resonant surface coils generating a low electric field in the proximity of their conductors. With this aim, we propose and experimentally demonstrate a leaky-wave antenna implemented as a periodically-slotted microstrip transmission line. Due to its non-resonant radiation, it induces only slightly over half the peak local SAR compared to a state-of-the-art dipole antenna but has the same transmit efficiency in prostate imaging at 7 T. Unlike other antennas for MRI, the leaky-wave antenna does not require to be tuned and matched when placed on a body, which makes it easy-to-use in prostate imaging at 7 T MRI.


Assuntos
Imageamento por Ressonância Magnética/instrumentação , Imagem Corporal Total/instrumentação , Absorção de Radiação , Radiação Eletromagnética , Desenho de Equipamento , Humanos , Campos Magnéticos/efeitos adversos , Imageamento por Ressonância Magnética/efeitos adversos , Imageamento por Ressonância Magnética/métodos , Imagens de Fantasmas , Imagem Corporal Total/efeitos adversos , Imagem Corporal Total/métodos
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